Mix&Go Advantages
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Mix&Go Advantages

Mix&Go takes the trial and error out of protein attachment.  With Mix&Go, activating a surface works reproducibly - every time.
The unique chemical characteristics of Mix&Go translate into a number of important advantages for the user:
  • Improved Sensitivity

    Damage-free attachment and proper orientation of antibodies on a surface result in greater assay sensitivity as every antibody on the surface will be capable of capturing antigens whereas with conventional attachment chemistries a significant portion of...

  • Reduced Antibody Usage

    Assay cost is often an important factor and antibodies can represent a significant portion of an assay’s cost. With Mix&Go the amount of antibody required to perform a given assay can be dramatically reduced. In standard microtiter plates and...

  • Damage-Free Attachment

    Existing methods of immobilisation, such as passive coating or covalent coupling, damage protein structure to varying degrees. Since damage to a protein’s three dimensional structure results in reduced or lost activity, preserving protein structure...

  • Rapid Binding Kinetics

    Fast kinetics with Mix&Go also means reduced time to result for your assays. Using Mix&Go coated beads we have been able to improve antibody as well as antigen binding kinetics significantly. Taken together these two factors allowed us to reduce...

  • Ease of Manufacturing

    Coating a surface with Mix&Go, an aqueous solution, is an easy and fast process. Generally, treating a surface, e.g. beads or slides with Mix&Go for 15 minutes at room temperature is sufficient. The coated surface has been shown to remain active...

  • Ease of Use

    Similarly activating a Mix&Go coated surface with the molecule of choice is a very easy process. Antibodies can be attached to a Mix&Go surface in 15 – 60 minutes at room temperature compared to a 16 hour procedure at 37oC for tosylated...

  • Improved Batch Uniformity

    Mix&Go is a polymeric coating and as such can mask inhomogeneities of the underlying surface to a high degree which greatly improves batch uniformity. Our work has shown that the %CV achieved by three operators simultaneously coupling 8 different...

  • Scalability

    Surface coating and protein attachment with Mix&Go can be easily scaled from the milligram to the gram scale. Therefore no change in procedure is required when transferring an assay from R&D to manufacturing – reducing cost and time.