Anteo Dx is specializing on the development proprietary surface coatings for the diagnostics and biotech industries. Our product, Mix&Go, represents a completely novel approach to coating synthetic surfaces and maintaining the function of these immobilized biomolecules. In addition, Anteo has developed a proprietary blocker and buffers to complement Mix&Go.
Attaching fragile proteins to a synthetic surface is a technique that is widely used in modern drug discovery research, life science R&D, and the development of therapeutic and diagnostic products. Depending on the particular method and application, many different synthetic materials - various plastics, glass, metals, and porous membranes - are used. The surface characteristics of these materials vary enormously. In addition, the type and number of different biomolecules that researchers need to immobilize is large, diverse and continually growing creating another major variable. Maintaining the functional activity of these diverse biomolecules on these diverse synthetic surfaces continues to be a challenge.
|Mix&Go™ uses a range of metal structures to determine binding location and overall binding strength.|
Mix&Go was designed to address this challenge by applying a successful principle found in nature: mussels need to attach themselves to rocks very securely so they will not get washed off. However, the attachment also has to be stretchy and flexible to dissipate the energy of the crashing waves. Mussels use multiple, firm but stretchy threads that can reform when broken to keep themselves firmly but flexibly attached.
Mix&Go is employing the same proven principle by acting like a double sided “molecular velcro” firmly yet flexibly binding biological molecules to synthetic surfaces.
Mix&Go is not one product but a family of proprietary metal polymers of different sizes and shapes. Each metal ion can form coordinate bonds with electron donating groups present on the vast majority of synthetic surfaces. This way Mix&Go can form a very strong attachment to the synthetic surface and use the remaining unoccupied coordination sites for binding of biological molecules.
All Mix&Go formulations are water based “glues” for cross-linking different materials, synthetic or otherwise. Depending on the formulation, Mix&Go products are selective for certain electron-binding motifs with a range of possible binding strengths. Current Mix&Go products were selected for strong binding, but if an application calls for some intermediate binding strength we can develop a Mix&Go specific for that purpose.
Mix&Go formulations can have selectivity to certain motifs of electron donating groups on the surface of biomolecules, e.g. we have selected a Mix&Go formulation that binds preferentially to the Fc region (the “stem” region) of antibodies therefore allowing for attachment of properly oriented and functionally active antibodies. Traditional methods tend to facilitate attachment on antibodies in a random orientation resulting in a large portion of damaged and/or non-functional antibodies.
The unique features make Mix&Go a true “one size fits all” surface chemistry, which can be used across the whole range of surfaces commonly employed in in vitro diagnostic testing and life science research.
Blockers and Buffers
Anteo has also developed a range of ancillary reagents for Mix&Go. The Block&Go solution is used to drastically reduce non-specific binding and a range of buffers and washing solutions ensure that Mix&Go works optimally.
To find out how the characteristics of Mix&Go translate into tangible benefits in the laboratory please refer to Mix&Go Advantages .